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Deshpande

Enzyme Immunoassays

From Concept to Product Development

Medium: Buch
ISBN: 978-0-412-05601-7
Verlag: Springer US
Erscheinungstermin: 30.06.1996
Lieferfrist: bis zu 10 Tage
Since the classical work of Rosalyn Yalow and Solomon Berson on the radioim­ munoassay of insulin in the late 1950s, which so brilliantly opened new domains in the diagnostics sector, immunoassays have been well received as a diagnostic and research tool in several disciplines of life sciences. The economics, rapidity, sensitivity, specificity, and the easy-to-use approach of immunodiagnostic tests are widely recognized, and have had a tremendous impact on clinical, agricultural, food, veterinary, and environmental diagnostics. At present, in the clinical diag­ nostic sector alone, immunoassay products command an annual worldwide mar­ ket value in excess of U. S. $10 billion, which is expanding by about 10% per annum. The recent trend toward increasing demand for automation of immunoassays and stable reagents clearly shows a definitive shift to nonisotopic immunoassay systems on a large scale. Historically, enzyme immunoassays have been in a prime position for in-house applications, primarily because of the easy access to inex­ pensive measuring equipment as well as to a vast body of literature on labeling techniques and applications. The recent popularity with the general public of sev­ eral over-the-counter immunoassay products based on this technology, such as glucose level kits for diabetics, cholesterol screening tests, and pregnancy detec­ tion test kits, is quite evident.

Produkteigenschaften


  • Artikelnummer: 9780412056017
  • Medium: Buch
  • ISBN: 978-0-412-05601-7
  • Verlag: Springer US
  • Erscheinungstermin: 30.06.1996
  • Sprache(n): Englisch
  • Auflage: 1996
  • Produktform: Gebunden, HC runder Rücken kaschiert
  • Gewicht: 1870 g
  • Seiten: 464
  • Format (B x H x T): 160 x 241 x 31 mm
  • Ausgabetyp: Kein, Unbekannt

Autoren/Hrsg.

Autoren

Deshpande, S. S.

Part 1: Basic considerations (concepts): Introduction. Antibody: biochemistry, structure, and function. Antigen: antibody reactions. Conjugation techniques. Antibody production. Enzymes and signal amplification systems. Separation and solid phase systems. Part 2: Product development: Immunoassay classification and commercial technologies. Assay development, evaluation, and validation. Reagent formulations and shelf life evaluation. Data analysis. Documentation, Registration, and Diagnostic Start-Ups.