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Kalish / Phillips

Clinical Applications of Capillary Electrophoresis

Methods and Protocols

Medium: Buch
ISBN: 978-1-62703-028-1
Verlag: Humana Press
Erscheinungstermin: 13.09.2012
Lieferfrist: bis zu 10 Tage
Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used.  Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science.  Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.

Produkteigenschaften


  • Artikelnummer: 9781627030281
  • Medium: Buch
  • ISBN: 978-1-62703-028-1
  • Verlag: Humana Press
  • Erscheinungstermin: 13.09.2012
  • Sprache(n): Englisch
  • Auflage: 2013
  • Serie: Methods in Molecular Biology
  • Produktform: Gebunden, HC gerader Rücken kaschiert
  • Gewicht: 816 g
  • Seiten: 311
  • Format (B x H x T): 183 x 260 x 21 mm
  • Ausgabetyp: Kein, Unbekannt

Autoren/Hrsg.

Herausgeber

Kalish, Heather

Phillips, Terry M.

Part I. Overview
 
1. An Overview of CE in Clinical Analysis
            David S. Hage
 
Part II. Applications in Clinical Chemistry
 
2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection
            Petr Tuma and Karel Štulik
 
3. The Selective Determination of Sulfates, Sulfonates and Phosphates in Urine by Capillary Electrophoresis – Mass Spectrometry
            Svenja-Catharina Bunz, and Christian Neusüß
 
4. Analysis of Neurotransmitter Amino Acids by CE-LIF Detection in Biological Fluids
            Angelo Zinellu, Salvatore Sotgia, Luca Deiana, and Ciriaco Carru
 
5. Expanded Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)
            Philip Britz-McKibbin
 
6. Development of an On-Site Measurement System for Salivary Stress-Related Substances Based on Microchip CE
            Yoshihide Tanaka and Nahoko Naruishi
 
7. Simultaneous Determination of Atenolol and Amiloride by CZE with Capacitively Coupled Conductivity Detection (C4D)
            Khaldun M. AL Azzam and Hassan Y. Aboul-Enein
 
8. A Homogeneous Immunoassay of Thyroxine Based on Microchip Electrophoresis and Chemiluminescence Detection
            Shulin Zhao and Yi-Ming Liu
 
9. High-Throughput Profiling of the Serum N-Glycome on Capillary Electrophoresis Microfluidics Systems
            Dieter Vanderschaeghe, Andras Guttman, and Nico Callewaert
 
10. Screening of Matrix Metalloproteinase Inhibitors by Microanalysis with Fluorescence Detection
            Xin Hai, Erwin Adams, and Ann Van Schepdael
 
Part III. Applications in Hematology
 
11. ABO Genotyping by Capillary Electrophoresis
            James Chun-I Lee, Hsing-Mei Hsieh, Hsiao-Feng Teng, and Li-Chin Tsai
 
12. Separation of Hemoglobin Variants by Capillary Electrophoresis
            Frédéric Cotton and Béatric Gulbis
 
13. Newborn Screening for Hemoglobinopathies Using Capillary Electrophoresis
            P.C. Giordano
 
Part IV. Applications in Bacteriology and Virology
 
14. Application of CZE in the Differentiation of Staphylococcus Aureus Strains
            Boguslaw Buszewski, Katarzyna Hrynkiewicz, and Ewelina Dziubakiewicz
 
15. Multiplex and Quantitative Pathogen Detection with High-Resolution Capillary Electrophoresis-Based Single Strand Conformation Polymorphism
            Hee Sung Hwang, Gi Won Shin, Boram Chung, Jeongkyeong Na, and Gyoo Yeol Jung
 
16. Application of CGE to Virus Identification
            Julia A. Fruetel and Victoria A. VanderNoot
 
Part V. Applications in Disease Biomarker Discovery
 
17. Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery
            Xueping Fang, Chenchen Wang, and Cheng S. Lee
 
18. Integrated Affinity and Electrophoresis Systems for Multiplexed Biomarker Analysis
            Pamela N. Nge, Jayson V. Pagaduan, Weichun Yang, and Adam T. Woolley
 
19. Urinary Proteomics Based on Capillary Electrophoresis Coupled to Mass Spectrometry in Kidney Disease
            Amaya Albalat, Julia Franke, Julien Gonzalez, Harald Mischak, and Petra Zürbig
 
20. Immunoaffinity, Capillary Electrophoresis, and Statistics for Studying Intact Alpha 1-Acid Glycoprotein Isoforms as Atherothrombosis Biomarker
            Angel Puerta, Pedro J. Martin-Alvarez, Sara Ongay, Jose C. Diez-Masa and  Mercedes de Frutos
 
Part VI. Applications in Immunology
 
21. Chip-Based Immunoassays
            Akwasi A. Apori and Amy E. Herr
 
22. CE Analysis of g-Globulin Mobility and Potential Clinical Utility      
            Dieter Vanderschaeghe, Evi Debruyne, Hans Van Vlierberghe, Nico Callewaert, and Joris Delanghe
 
23. Cytokine Analysis by Immunoaffinity Capillary Electrophoresis
            Mark Mendonca and Heather Kalish
 
Part VII. Applications in Clinical Genetic Analysis
 
24. Capillary Electrophoresis